[Immunogenic properties of recombinant vaccinia virus containing the human IL-2 gene].
Vopr Virusol
; 53(1): 27-31, 2008.
Article
en Ru
| MEDLINE
| ID: mdl-18318132
ABSTRACT
A genetic construct of the human interleukin-2 (IL-2) gene within vaccinia virus (L-IVP strain) has been designed. The authors show the capacity of CV-1 cells infected with the recombinant vaccinia virus VV-SIL2 to secrete human IL-2 into the culture medium. Human IL-2 has been detected by immunoblotting. The sera from the animals immunized with the recombinant virus VV-SIL2 exhibited both human IL-2 and its antibodies throughout the observation period. This recombinant virus immunization induced both humoral and cell-mediated immune responses to human IL-2; the observed changes in the concentrations of cytokines are likely to suggest that the response predominantly followed a Th1 pathway. The study construct was nontoxic at the used concentrations and administration routes. The findings point that it is promising to investigate the adjuvant properties of the recombinant VV-SIL2 vaccine-based preparation for immunization in combination with various vaccines and to study this construct in therapy for cancer diseases.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
2_ODS3
/
4_TD
Problema de salud:
2_enfermedades_transmissibles
/
4_smallpox
Asunto principal:
Virus Vaccinia
/
Vacuna contra Viruela
/
Inmunización
/
Interleucina-2
/
Infecciones por Poxviridae
/
Anticuerpos Antivirales
Límite:
Animals
/
Humans
/
Male
Idioma:
Ru
Revista:
Vopr Virusol
Año:
2008
Tipo del documento:
Article