Isolation of membrane protein complexes by blue native electrophoresis.
Methods Mol Biol
; 424: 423-31, 2008.
Article
en En
| MEDLINE
| ID: mdl-18369880
Blue native PAGE is a discontinuous electrophoretic system that allows the separation of membrane protein complexes in a native, enzymatically active state with high resolution. Membrane protein complexes are solubilized by neutral, nondenaturing detergents like n-dodecyl-beta-D-maltoside. After addition of Coomassie G250 that binds to the surface of the proteins, separation of the negatively charged complexes according to molecular mass is possible. After electrophoresis the structure and function of the isolated protein complexes can be investigated.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Colorantes de Rosanilina
/
Complejos Multiproteicos
/
Electroforesis en Gel de Poliacrilamida
/
Glucósidos
/
Proteínas de la Membrana
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2008
Tipo del documento:
Article
País de afiliación:
Alemania