Studies on the adaptive response in mouse female germ cells X-irradiated in vitro at two different stages of maturation.
In Vivo
; 22(2): 179-86, 2008.
Article
en En
| MEDLINE
| ID: mdl-18468401
BACKGROUND: Radioadaptation is a phenomenon whereby cells exposed to a low dose of ionizing radiation are more resistant to a much higher dose delivered some time thereafter. This phenomenon could result from the activation of damage repair and/or antioxidant defense systems by the low dose. MATERIALS AND METHODS: The existence of a cytogenetic adaptive response in female germ cells was investigated using a recently developed in vitro system. Mouse ovarian follicles were cultured from an early preantral stage up to ovulation. The follicles were X-irradiated with either 2 or 4 Gy ("challenge dose") preceded or not by 50 mGy ("conditioning dose", 5 h earlier), on days 0 or 12 of the culture. Ovulated oocytes were collected on day 13, fixed and analyzed for the presence of chromosome aberrations. RESULTS: Irradiation with 2 or 4 Gy on days 0 or 12 did not influence ovulation but had dose-dependent effects on the germinal vesicle breakdown of the oocytes. It also caused dose-dependent chromosome damage, with a greater sensitivity of oocytes to this effect when irradiation occurred on day 12 than on day 0. Prior irradiation of oocytes with the dose of 50 mGy led to a reduction in the yield of chromosome aberrations when irradiation occurred on day 12 but not on day 0. CONCLUSION: These results suggest that pre-irradiation of mouse pre-ovulatory oocytes with a low conditioning dose could confer on them some protection against radiation-induced chromosomal damage by a subsequent challenge dose of a few Gy.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oocitos
/
Adaptación Fisiológica
/
Células Germinativas
Límite:
Animals
Idioma:
En
Revista:
In Vivo
Asunto de la revista:
NEOPLASIAS
Año:
2008
Tipo del documento:
Article
País de afiliación:
Bélgica