Hoechst 33258--poly(dG-dC).poly(dG-dC) complexes of three types.
J Biomol Struct Dyn
; 26(1): 99-114, 2008 Aug.
Article
en En
| MEDLINE
| ID: mdl-18533731
ABSTRACT
It was found recently that Hoechst 33258, a dsDNA fluorescent dye used in cytological studies, is an efficient inhibitor of the interaction of TATA-box-binding protein with DNA, DNA topoisomerase I, and DNA helicases. In addition it proved to be a radioprotector. Biological activity of Hoechst 33258 may be associated with dsDNA complexes of not only monomeric, but also dimeric type. In this work, the Hoechst 33258 interaction with poly(dG-dC).poly(dG-dC) was studied using UV-vis and fluorescent spectroscopy, circular and flow-type linear dichroism. It was found that Hoechst 33258 formed with poly(dG-dC).poly(dG-dC) complexes of three types, namely, monomeric, dimeric, and, apparently, tetrameric, and their spectral properties were studied. Complexes of monomeric and dimeric types competed with distamycin A, a minor groove ligand, for binding to poly(dG-dC).poly(dG-dC). We proposed that Hoechst 33258 both monomers and dimers form complexes of the external type with poly(dG-dC).poly(dG-dC) from the side of the minor groove.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Poli C
/
Poli G
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Polinucleótidos
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ADN-Topoisomerasas de Tipo I
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Colorantes Fluorescentes
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Bisbenzimidazol
Idioma:
En
Revista:
J Biomol Struct Dyn
Año:
2008
Tipo del documento:
Article
País de afiliación:
Rusia