Studies on diuretic and hypouricemic effects of Orthosiphon stamineus methanol extracts in rats.
J Ethnopharmacol
; 118(3): 354-60, 2008 Aug 13.
Article
en En
| MEDLINE
| ID: mdl-18602231
ABSTRACT
AIM OF THE STUDY Orthosiphon stamineus (Labiatae) is a traditional folk medicine widely used in Southeast Asia for the treatment of several kidney disorders, gout and as a diuretic. This study was conducted to examine the diuretic and hypouricemic effects of Orthosiphon stamineus leaf extracts. MATERIALS AND METHODS:
The diuretic effect of different methanol extracts was examined by treating different groups of Sprague-Dawley rats with single (2g/kg) oral doses of methanol and methanolwater (11) extracts. Hydrochlorothiazide (10mg/kg) was used as positive control in acute study. Methanol and methanol water (11) extracts at 0.5 g/kg were administered for a period of 7 consecutive days. Cumulative urine volume and electrolytes (Na+ and K+) concentrations at different time intervals were measured. On the other hand, hypouricemic activity of methanolwater extract (11) was experimented using different oral single doses (0.25, 0.5, 1 and 2g/kg). Allopurinol was used as positive control. Uric acid concentration in serum was analyzed by using RP-HPLC at 280 nm.RESULTS:
Sodium and potassium excretion increased significantly (p<0.05 and <0.01) in the first 8h of treatment with a single dose (2g/kg) of the extracts in a pattern comparable to that of the known diuretic hydrochlorothiazide. Meanwhile, repeated administration of 0.5 g/kg methanolwater (11) extract showed a significant increase in urine volume (from day 3 to day 7) (p<0.01) and electrolytes excretion (Na+ and K+) from day 2 to day 7 (p<0.05 and <0.01). On the other hand, 0.5, 1 and 2g/kg of methanolwater (11) extract and the standard allopurinol reduced the serum urate level in hyperuricemic rats at hour 6.CONCLUSION:
These results provided an evidence of the high tendency of methanolwater (11) extract of Orthosiphon stamineus towards diuretic and hypouricemic effects in rats.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácido Úrico
/
Extractos Vegetales
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Orthosiphon
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Diuréticos
Límite:
Animals
Idioma:
En
Revista:
J Ethnopharmacol
Año:
2008
Tipo del documento:
Article
País de afiliación:
Malasia