Assessment of the nitrosylation process.
Curr Opin Clin Nutr Metab Care
; 11(5): 587-91, 2008 Sep.
Article
en En
| MEDLINE
| ID: mdl-18685454
ABSTRACT
PURPOSE OF REVIEW To understand the principles and limits of the methodologies used for the measurement of S-nitrosylated proteins. RECENT FINDINGS:
Among methods for studying protein S-nitrosylation, chemoluminescence and biotin switch assay have rapidly gained popularity. However, recent findings have attempted to highlight potential pitfalls for these methods. Many assays for biological S-nitrosylated proteins are used near the limit of detection and pretreatment of the biological samples can modify the S-NO bond. These results suggest that additional controls are essential in order to identify S-nitrosylated proteins and results should be quantitatively validated using more than one methodology.SUMMARY:
Protein S-nitrosylation is emerging as a key mechanism by which nitric oxide regulates cell signalling. This review focuses on existing methodologies for the measurement of S-nitrosylated proteins in biological matrices and the potential pitfalls of each method.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas
/
Óxido Nítrico
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Curr Opin Clin Nutr Metab Care
Asunto de la revista:
CIENCIAS DA NUTRICAO
/
METABOLISMO
Año:
2008
Tipo del documento:
Article
País de afiliación:
Francia