Effect of blood sample handling post-collection on Erysipelothrix rhusiopathiae antibody titres.
Vet J
; 180(3): 325-9, 2009 Jun.
Article
en En
| MEDLINE
| ID: mdl-18783968
ABSTRACT
A study was conducted to determine the effect of blood sample mishandling on the performance of an enzyme-linked immunosorbent assay for the detection of antibodies against Erysipelothrix rhusiopathiae. Eleven sample maltreatments (storage at -10 degrees C, storage at 4 degrees C, heat treatment of clotted blood, haemolysis, repetitive freeze-thaw cycling, and substitution of plasma in place of serum) were simulated in a laboratory environment and then run concurrently against a gold standard sample (storage at -80 degrees C). The mishandling treatment groups that simulated high levels of haemolysis had significantly lower optical density (OD) readings when compared to the gold standard. However, the magnitude of the effects was relatively small and only samples with OD values close to the cut-off changed state from positive to negative. Heat treatment had a minor, but non-significant, effect on OD values. Findings from this study suggested that immunoglobulin G antibody was stable in the face of most common sample mishandling events.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Manejo de Especímenes
/
Erysipelothrix
/
Infecciones por Erysipelothrix
/
Anticuerpos Antibacterianos
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Vet J
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Nueva Zelanda