Activation of the ERK signal transduction pathway by Epstein-Barr virus immediate-early protein Rta.
J Gen Virol
; 89(Pt 10): 2437-2446, 2008 Oct.
Article
en En
| MEDLINE
| ID: mdl-18796711
ABSTRACT
BRCA1-associated protein 2 (BRAP2) is known to interact with the kinase suppressor of Ras 1 (KSR1), inhibiting the ERK signal transduction cascade. This study found that an Epstein-Barr virus (EBV) immediate-early protein, Rta, is a binding partner of BRAP2 in yeast and confirmed the binding in vitro by a glutathione S-transferase pull-down assay and in vivo by co-immunoprecipitation in 293(maxi-EBV) cells. Binding studies also showed that Rta and KSR1 interacted with the C-terminal 202 aa region in BRAP2. Additionally, Rta appeared to prevent the binding of KSR1 to BRAP2, activating the ERK signal transduction pathway and the transcription of an EBV immediate-early gene, BZLF1. Activation of the ERK signal transduction pathway by Rta may be critical for the maintenance of the lytic state of EBV.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transducción de Señal
/
Activación Transcripcional
/
Transactivadores
/
Proteínas Inmediatas-Precoces
/
Herpesvirus Humano 4
/
Quinasas MAP Reguladas por Señal Extracelular
Límite:
Humans
Idioma:
En
Revista:
J Gen Virol
Año:
2008
Tipo del documento:
Article