DNA at aqueous/solid interfaces: chirality-based detection via second harmonic generation activity.
J Am Chem Soc
; 131(2): 844-8, 2009 Jan 21.
Article
en En
| MEDLINE
| ID: mdl-19140801
ABSTRACT
We present electronic spectra of single-strand and duplex DNA oligonucleotides covalently attached to fused quartz/aqueous interfaces and demonstrate that a strong nonlinear optical linear dichroism response is obtained when adenine and thymine bases undergo Watson-Crick base pairing to form a double helix. Complementary chi(3) charge screening studies indicate that the signal originates from 5 x 10(11) strands per square centimeter, or 6 attomoles of surface-bound oligonucleotides. The label-free, molecular-specific nature afforded by nonlinear optical studies of DNA at aqueous/solid interfaces allows for the real-time tracking of interfacial DNA hybridization for the first time.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cuarzo
/
ADN
/
Agua
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Am Chem Soc
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos