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Immunodiagnosis of human neurocysticercosis using a synthetic peptide selected by phage-display.
Hell, R C R; Amim, P; de Andrade, H M; de Avila, R A M; Felicori, L; Oliveira, A G; Oliveira, C A; Nascimento, E; Tavares, C A P; Granier, C; Chávez-Olórtegui, C.
Afiliación
  • Hell RC; Departamento de Bioquímica-Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte-MG, Brazil.
Clin Immunol ; 131(1): 129-38, 2009 Apr.
Article en En | MEDLINE | ID: mdl-19186111
The usefulness of a synthetic peptide in the serodiagnosis of Taenia solium human neurocysticercosis (NC) has been evaluated. Phage-displayed peptides were screened with human antibodies to scolex protein antigen from cysticercus cellulosae (SPACc). One clone was found to interact specifically with anti-SPACc IgGs. The corresponding synthetic peptide was found to be recognized in ELISA by NC patient's sera. The study was carried out with sera from 28 confirmed NC patients, 13 control sera and 73 sera from patients suffering from other infectious diseases. A 93% sensibility and a 94.3% specificity was achieved. Figures of 89% and 31.4% of sensibility and specificity were obtained in a SPACc-based ELISA. Immunoblotting of SPACc with anti-peptide antibodies revealed a single band of approximately 45 kDa in 1D and four 45 kDa isoforms in 2D-gel electrophoresis. A strong and specific immunostaining in the fibers beneath the suckers, at the base of the rostellum, and in the tissue surrounding the scolex of cysticerci was observed by immunomicroscopy. Our results show that a peptide-based immunodiagnostic of neurocisticercosis can be envisioned.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 2_ODS3 / 3_ND Problema de salud: 2_enfermedades_transmissibles / 3_helminthiasis / 3_neglected_diseases / 3_zoonosis Asunto principal: Péptidos / Pruebas Inmunológicas / Anticuerpos Antihelmínticos / Neurocisticercosis / Taenia solium / Antígenos Helmínticos Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Clin Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2009 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 2_ODS3 / 3_ND Problema de salud: 2_enfermedades_transmissibles / 3_helminthiasis / 3_neglected_diseases / 3_zoonosis Asunto principal: Péptidos / Pruebas Inmunológicas / Anticuerpos Antihelmínticos / Neurocisticercosis / Taenia solium / Antígenos Helmínticos Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Clin Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2009 Tipo del documento: Article País de afiliación: Brasil
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