Expression of matrix metalloproteinases (MMP)-12 by myofibroblasts during alkali-burned corneal wound healing.
Curr Eye Res
; 34(3): 207-14, 2009 Mar.
Article
en En
| MEDLINE
| ID: mdl-19274528
ABSTRACT
PURPOSE:
The purpose of this study was to determine the expression of MMP-12 by myofibroblasts during the healing of alkali-burned rabbit corneas (ARC), thus implicating its role in ECM remodeling.METHODS:
Rabbit corneas during alkali burn were examined for MMP-12 mRNA expression by RT-PCR. Immunohistochemistry was used to determine the presence of alpha-SMA, MMP-12 protein, and macrophages. In situ hybridization was performed to identify MMP-12 mRNA expressing cells.RESULTS:
RT-PCR showed that MMP-12 mRNA was expressed in the alkali-burned corneas from one week after the injury. Immunohistochemistry showed myofibroblasts positive for MMP-12 expression. In situ hybridization revealed that MMP-12 mRNA was expressed by myofibroblasts.CONCLUSION:
Our results indicate that, in alkali-burned corneas, myofibroblasts express both MMP-12 mRNA and protein. We suggest that MMP-12 may disintegrate some components of the ECM released after severe alkali burn, which may be involved in the ECM remodeling.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cicatrización de Heridas
/
Quemaduras Químicas
/
Regulación Enzimológica de la Expresión Génica
/
Quemaduras Oculares
/
Córnea
/
Metaloproteinasa 12 de la Matriz
/
Fibroblastos
Límite:
Animals
Idioma:
En
Revista:
Curr Eye Res
Año:
2009
Tipo del documento:
Article
País de afiliación:
Japón