Leukocyte activation and circulating leukocyte-derived microparticles in preeclampsia.
Am J Reprod Immunol
; 61(5): 346-59, 2009 May.
Article
en En
| MEDLINE
| ID: mdl-19341385
ABSTRACT
PROBLEM:
Preeclampsia shows characteristics of an inflammatory disease including leukocyte activation. Analyses of leukocyte-derived microparticles (MP) and mRNA expression of inflammation-related genes in leukocytes may establish which subgroups of leukocytes contribute to the development of preeclampsia. METHOD OF STUDY Blood samples were obtained from preeclamptic patients, normotensive pregnant and non-pregnant controls. sL-selectin and elastase were measured by ELISA. mRNA was isolated from leukocytes and gene expression was determined by multiplex ligation-dependent probe amplification (MLPA). MP were characterized by flow cytometry.RESULTS:
Altered concentrations of sL-selectin and elastase confirmed leukocyte activation in preeclampsia. These leukocytes showed up-regulation of Nuclear Factor of Kappa light chain gene enhancer in B cells inhibitor (NFkappaB-1A) and cyclin-dependent kinase inhibitor (CDKN)-1A compared with normotensive pregnant women. Interleukin-1 Receptor Antagonist (IL-1RA) and tumor necrosis factor (TNF)-R1 were increased compared with those in non-pregnant controls. Monocyte-derived MP were elevated in preeclamptic patients compared with pregnant women. The numbers of cytotoxic T-cell-derived and granulocyte-derived MP were elevated compared with those of non-pregnant women.CONCLUSION:
Leukocytes are activated in preeclampsia. A pro-inflammatory gene expression profile is not prominent, although differences in mRNA expression can be detected. Increased levels of particular subsets of leukocyte-derived MP reflect activation of their parental cells in preeclampsia.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Preeclampsia
/
Activación de Linfocitos
/
Micropartículas Derivadas de Células
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Inflamación
/
Leucocitos
Límite:
Adult
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Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Am J Reprod Immunol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Países Bajos