Quantitative detection of Plasmodium falciparum DNA in saliva, blood, and urine.
J Infect Dis
; 199(11): 1567-74, 2009 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-19432544
ABSTRACT
BACKGROUND:
Current methods for detecting malaria parasites are invasive and associated with poor compliance when repeated sampling is required. New methods to detect and quantify parasites in a less-invasive manner would greatly enhance the potential for longitudinal surveillance in clinical trials.METHODS:
Saliva, urine, and blood samples from 386 Gambian outpatients with suspected malaria infections were analyzed by nested polymerase chain reaction (nPCR) to detect infection and to evaluate diagnostic accuracy in comparison to expert microscopy. The amount of parasite DNA in malaria-positive samples was estimated using real-time quantitative PCR (qPCR).RESULTS:
Blood parasite density as estimated by qPCR correlated well with parasite counts established by microscopy (p = 0.94; P < .001). qPCR results for saliva had a significant correlation with microscopy counts (p = 0.58; P < .001), whereas qPCR results for urine had a positive but poor correlation with microscopy counts (p = 0.20; P = .117). The mean amounts of parasite DNA quantified in blood were greater than the mean amounts quantified in saliva and urine samples obtained concurrently from the same individual, by approximately 600-fold and approximately 2500-fold, respectively. When nPCR results were compared with microscopy results, nPCR of saliva had a sensitivity of 73% and a specificity of 97%; its sensitivity increased to 82% in samples with a parasite density of > or = 1000 parasites/microL. nPCR of urine had a sensitivity of 32% and a specificity of 98%.CONCLUSION:
Saliva sampling is a promising less-invasive approach for detecting malaria infection.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_malaria
/
3_neglected_diseases
Asunto principal:
Plasmodium falciparum
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ADN Protozoario
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Malaria
Tipo de estudio:
Diagnostic_studies
Límite:
Adolescent
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Adult
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Animals
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Child
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Humans
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Middle aged
Idioma:
En
Revista:
J Infect Dis
Año:
2009
Tipo del documento:
Article