Identification of novel genes putatively involved in the photosystem synthesis of Bradyrhizobium sp. ORS 278.
Photosynth Res
; 100(2): 97-105, 2009 May.
Article
en En
| MEDLINE
| ID: mdl-19452262
ABSTRACT
In aerobic anoxygenic phototrophs, oxygen is required for both the formation of the photosynthetic apparatus and an efficient cyclic electron transfer. Mutants of Bradyrhizobium sp. ORS278 affected in photosystem synthesis were selected by a bacteriochlorophyll fluorescence-based screening. Out of the 9,600 mutants of a random Tn5 insertion library, 50 clones, corresponding to insertions in 28 different genes, present a difference in fluorescence intensity compared to the WT. Besides enzymes and regulators known to be involved in photosystem synthesis, 14 novel components of the photosynthesis control are identified. Among them, two genes, hsIU and hsIV, encode components of a protein degradation complex, probably linked to the renewal of photosystem, an important issue in Bradyrhizobia which have to deal with harmful reactive oxygen species. The presence of homologs in non-photosynthetic bacteria for most of the regulatory genes identified during study suggests that they could be global regulators, as the RegA-RegB system.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bradyrhizobium
/
Proteínas del Complejo del Centro de Reacción Fotosintética
/
Genes Bacterianos
/
Mutación
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Photosynth Res
Asunto de la revista:
METABOLISMO
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia