Involvement of Pta1, Pcf11 and a KlCYC1 AU-rich element in alternative RNA 3'-end processing selection in yeast.

ABSTRACT

This work reports the involvement of yeast RNA processing factors Pta1 and Pcf11 in alternative 3'-end RNA processing. The pta1-1 and pcf11-2 mutations changed the predominance of KlCYC1 1.14 and 1.5 kb transcript isoforms. Mutation of the KlCYC1 3'-UTR AU-rich sequence at positions 679-690 (mutant M1) altered transcript predominance. Moreover, expression of M1 in the yeast mutants partially suppressed their effects in the predominance pattern. The combination of the M1 and M2 (694-698 deletion) mutations abolished the alternative processing. Pta1 involvement in this selection was confirmed using the Pta1-td degron strain.