Involvement of Pta1, Pcf11 and a KlCYC1 AU-rich element in alternative RNA 3'-end processing selection in yeast.
FEBS Lett
; 583(17): 2843-8, 2009 Sep 03.
Article
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| MEDLINE
| ID: mdl-19646984
ABSTRACT
This work reports the involvement of yeast RNA processing factors Pta1 and Pcf11 in alternative 3'-end RNA processing. The pta1-1 and pcf11-2 mutations changed the predominance of KlCYC1 1.14 and 1.5 kb transcript isoforms. Mutation of the KlCYC1 3'-UTR AU-rich sequence at positions 679-690 (mutant M1) altered transcript predominance. Moreover, expression of M1 in the yeast mutants partially suppressed their effects in the predominance pattern. The combination of the M1 and M2 (694-698 deletion) mutations abolished the alternative processing. Pta1 involvement in this selection was confirmed using the Pta1-td degron strain.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Procesamiento Postranscripcional del ARN
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Regiones no Traducidas 3'
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Proteínas de Saccharomyces cerevisiae
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Factores de Escisión y Poliadenilación de ARNm
Idioma:
En
Revista:
FEBS Lett
Año:
2009
Tipo del documento:
Article
País de afiliación:
España