Intracellular presence of insulin and its phosphorylated receptor in non-small cell lung cancer.
J Cell Physiol
; 221(3): 766-70, 2009 Dec.
Article
en En
| MEDLINE
| ID: mdl-19688775
ABSTRACT
Insulin has been known for a long time to influence the growth and differentiation of normal and transformed cells. In order to delineate the role of insulin specifically in non-small cell lung cancer (NSCLC), we have now searched by immunohistochemistry (IHC) for the presence of insulin in NSCLC samples. Among the 112 samples we studied, 30 were found to contain insulin, which was detected in the form of intracytoplasmic granula. Moreover, its expression significantly correlated with (a) the morphological/histopathological subtype of NSCLC, being more frequent in adenocarcinomas; (b) the grade of tumor differentiation, displaying an increase in low-grade carcinomas; (c) tumor size, occurring predominantly in smaller tumors; (d) the presence of phosphorylated, activated insulin receptor; (e) the median patient age, being present in relatively younger individuals. Furthermore and interestingly, surrounding atypical adenomatous hyperplastic areas and normal alveolar pneumocytes scored insulin-positive in some of the insulin-negative tumors. In addition, PCR exploration for insulin transcripts in some samples positive for immunoreactive insulin was negative, indicating a possibly exogenous origin for the intracellular insulin in our NSCLC cohort. Taken together, our data suggest that an intracellular insulin activity is important for the progression of low-grade human lung adenocarcinomas.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Receptor de Insulina
/
Antígenos CD
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Carcinoma de Pulmón de Células no Pequeñas
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Espacio Intracelular
/
Insulina
Límite:
Aged
/
Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
J Cell Physiol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Italia