The African swine fever virus g5R protein possesses mRNA decapping activity.
Virology
; 393(1): 177-82, 2009 Oct 10.
Article
en En
| MEDLINE
| ID: mdl-19695654
ABSTRACT
The African Swine Fever Virus (ASFV) encodes a single Nudix enzyme in its genome, termed the g5R protein (g5Rp). Nudix phosphohydrolases cleave a variety of substrates, such as nucleotides and diphosphoinositol polyphosphates. Previously, ASFV g5Rp was shown to hydrolyze diphosphoinositol polyphosphates and GTP, but was unable to cleave methylated mRNA cap analogues. In vaccinia virus (VACV), a distant relative of ASFV, the D9 and D10 Nudix enzymes were shown to cleave the mRNA cap, but only when the cap was attached to an RNA body. Here, we show that recombinant ASFV g5Rp hydrolyzes the mRNA cap when tethered to an RNA moiety, liberating m(7)GDP as a product. Mutations in the Nudix motif abolished mRNA decapping activity, confirming that g5Rp was responsible for cap cleavage. The decapping activity of g5Rp was potently inhibited by excess uncapped RNA but not by methylated cap analogues, suggesting that substrate recognition occurs by RNA binding.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pirofosfatasas
/
Proteínas Virales
/
ARN Mensajero
/
Virus de la Fiebre Porcina Africana
/
Endorribonucleasas
Idioma:
En
Revista:
Virology
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos