[Production of marker-free plants expressing the gene of the hepatitis B virus surface antigen].
Genetika
; 45(8): 1055-60, 2009 Aug.
Article
en Ru
| MEDLINE
| ID: mdl-19769294
ABSTRACT
The pBM plasmid, carrying the gene of hepatitis B virus surface antigen (HBsAg) and free of any selection markers of antibiotic or herbicide resistance, was constructed for genetic transformation of plants. A method for screening transformed plant seedlings on nonselective media was developed. Enzyme immunoassay was used for selecting transgenic plants with HBsAg gene among the produced regenerants; this method provides for a high sensitivity detection of HBsAg in plant extracts. Tobacco and tomato transgenic lines synthesizing this antigen at a level of 0.01-0.05% of the total soluble protein were obtained. The achieved level of HBsAg synthesis is sufficient for preclinical trials of the produced plants as a new generation safe edible vaccine. The developed method for selecting transformants can be used for producing safe plants free of selection markers.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Nicotiana
/
Virus de la Hepatitis B
/
Solanum lycopersicum
/
Antígenos de Superficie de la Hepatitis B
Idioma:
Ru
Revista:
Genetika
Año:
2009
Tipo del documento:
Article