Improved rapid assay of plasma uric acid by short-end injection capillary zone electrophoresis.
Anal Bioanal Chem
; 395(8): 2577-82, 2009 Dec.
Article
en En
| MEDLINE
| ID: mdl-19806347
ABSTRACT
A rapid and simple short-end injection capillary zone electrophoresis method was developed for the quantification of plasma uric acid. The separation was performed in an uncoated fused-silica capillary (50 microm ID, 60 cm total length, 10.2 cm effective length) by using as a background electrolyte a 75 mmol/L glycylglycine solution titrated with NaOH 5 mol/L to pH 9.0, a voltage of 28 kV, a cartridge temperature of 15 degrees C, and direct UV detection at 292 nm. Under optimized conditions, uric acid was determinate in little more than 1 min (1.076 minutes). In order to verify the accuracy of the analysis, urate levels were measured in 543 apparently healthy volunteers by the new assay and our previous method, and the obtained data were compared by Passing-Bablock regression, Bland-Altman test, and a new regression-based approach, which showed a good agreement between two methods.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bioensayo
/
Electroforesis Capilar
Límite:
Humans
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2009
Tipo del documento:
Article
País de afiliación:
Italia