Remodeling of phosphatidylglycerol in Synechocystis PCC6803.
Biochim Biophys Acta
; 1801(2): 163-70, 2010 Feb.
Article
en En
| MEDLINE
| ID: mdl-19857602
ABSTRACT
The phosphatidylglycerol deficient DeltapgsA mutant of Synechocystis PCC6803 provided a unique experimental system for investigating in vivo retailoring of exogenously added dioleoylphosphatidylglycerol in phosphatidylglycerol-depleted cells. Gas chromatographic analysis of fatty acid composition suggested that diacyl-phosphatidylglycerols were synthesized from the artificial synthetic precursor. The formation of new, retailored lipid species was confirmed by negative-ion electrospray ionization-Fourier-transform ion cyclotron resonance and ion trap tandem mass spectrometry. Various isomeric diacyl-phosphatidylglycerols were identified indicating transesterification of the exogenously added dioleoylphosphatidyl-glycerol at the sn-1 or sn-2 positions. Polyunsaturated fatty acids were incorporated selectively into the sn-1 position. Our experiments with Synechocystis PCC6803/DeltapgsA mutant cells demonstrated lipid remodeling in a prokaryotic photosynthetic bacterium. Our data suggest that the remodeling of diacylphosphatidylglycerol likely involves reactions catalyzed by phospholipase A(1) and A(2) or acyl-hydrolase, lysophosphatidylglycerol acyltransferase and acyl-lipid desaturases.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfatidilgliceroles
/
Synechocystis
/
Ácidos Grasos Insaturados
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
2010
Tipo del documento:
Article
País de afiliación:
Hungria