Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss).
Comp Biochem Physiol C Toxicol Pharmacol
; 151(2): 209-15, 2010 Mar.
Article
en En
| MEDLINE
| ID: mdl-19883795
ABSTRACT
In this study we examined gene expression of a series of ABC efflux transporters in various rainbow trout (Oncorhynchus mykiss) tissues. Based on their reported toxicological relevance, we have used quantitative real time PCR SYBR green quantification methodology, with combination of absolute and relative approach, to quantify RNA expression of eight ABC transporters from three different families abcb1 and abcb11, abcc1-5 and abcg2. Level of mRNA transcripts was measured in seven tissues liver, brain, gonads, kidney, gills, proximal intestine and distal intestine, and the obtained expression profiles were compared with data available for related mammalian tissues. Most of the analyzed genes showed similar gene expression pattern as the ones found in mammals, with some notable exceptions. E.g., gills were in our study characterized by extremely low expression of all analyzed ABC genes; and despite the pronounced role of ABCC1 (MRP1) in protection of mammalian cells from chemical toxins, we found low expression of this transporter in trout tissues. Taken together, our study offers the first thorough insight into distribution pattern of (eco)toxicologically relevant ABC transporters, serving as a necessary base for further studies directed to better understanding of physiological and/or protective role of ABC transporters in fish.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oncorhynchus mykiss
/
Transportadoras de Casetes de Unión a ATP
/
Perfilación de la Expresión Génica
/
Proteínas de Peces
Límite:
Animals
Idioma:
En
Revista:
Comp Biochem Physiol C Toxicol Pharmacol
Asunto de la revista:
FARMACOLOGIA
/
TOXICOLOGIA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Croacia