Chelex-based DNA isolation procedure for the identification of microbial communities of eggshell surfaces.
Anal Biochem
; 397(2): 253-5, 2010 Feb 15.
Article
en En
| MEDLINE
| ID: mdl-19887062
ABSTRACT
Microbial communities present on eggshell surfaces of wild birds are weakly studied, especially their influence on embryo infection and, thus, egg viability. Bacterial density of wild bird eggshells is very low, and most DNA extraction protocols are frequently unsuccessful. We have efficiently adapted a chelex-based DNA isolation method for 16S ribosomal gene amplification from the total communities of eggshell surfaces from six avian species.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aves
/
ADN Bacteriano
/
Cáscara de Huevo
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Anal Biochem
Año:
2010
Tipo del documento:
Article
País de afiliación:
España