Amperometric glucose biosensor based on biocompatible poly(dimethylaminoethyl) methacrylate microparticles.
Talanta
; 81(4-5): 1197-202, 2010 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-20441884
ABSTRACT
Glucose oxidase (GOx) has been immobilized within poly(dimethylamino) ethyl methacrylate (p-DMAEM) microparticles which were subsequently used as biological material in the fabrication of a glucose biosensor. The enzyme immobilization method was optimized in relation with the monomer concentration and cross-linker content. It was found that the best biosensor response corresponds to microparticles synthesized with 1.19M monomer and 0.37% cross-linking content. Furthermore, the influence on the biosensor response of parameters such as working potential, pH, temperature, and loaded enzyme were investigated. In addition, analytical properties such as sensitivity, linear range, response time, and detection limit were determined. The biosensor was used to analyze glucose in human serum samples with satisfactory results. The useful lifetime of the biosensor is at least 520 days.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polímeros
/
Glucemia
/
Técnicas Biosensibles
/
Automonitorización de la Glucosa Sanguínea
/
Electroquímica
/
Metacrilatos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Talanta
Año:
2010
Tipo del documento:
Article
País de afiliación:
España