HIV-1 Gag p24-Nef fusion peptide induces cellular and humoral immune response in a mouse model.
Acta Virol
; 54(2): 131-6, 2010.
Article
en En
| MEDLINE
| ID: mdl-20545443
ABSTRACT
UNLABELLED Many Human immunodeficiency virus (HIV) candidate vaccines have been tested in clinical trials, but none was sufficiently effective in the prevention of HIV infection. A HIV vaccine should induce humoral as well as cell-mediated response, the latter including the cytotoxic CD8+ T lymphocyte (CTL) response. In this study, we immunized BALB/c mice with a purified fusion peptide Gag p24-Nef and evaluated immune responses. As for the cellular responses, the adjuvanted fusion peptide induced lymphocyte proliferation, CTL response, and cytokines IFN-gamma and IL-4 in the Th1 pattern. Humoral immune response to the adjuvanted fusion peptide included an increase in IgG antibodies of more IgG2a than IgG1 subtype. These results indicate that the employed HIV-1 peptide construct can elicit both cellular and humoral immune responses in mice. Further studies aimed at memory T cells and other aspects of immune responses are needed before a comprehensive assessment of this candidate vaccine could be provided. KEYWORDS epitopes; fusion peptide; HIV-1 p24-Nef; immune response.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
VIH-1
/
Proteína p24 del Núcleo del VIH
/
Vacunas contra el SIDA
/
Productos del Gen nef del Virus de la Inmunodeficiencia Humana
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Acta Virol
Año:
2010
Tipo del documento:
Article
País de afiliación:
Irán