Deletion of a prophage-like element causes attenuation of Salmonella enterica serovar Enteritidis and promotes protective immunity.
Vaccine
; 28(33): 5458-66, 2010 Jul 26.
Article
en En
| MEDLINE
| ID: mdl-20558245
ABSTRACT
Salmonella enterica serovar Enteritidis (S. Enteritidis) is a wide host range serovar belonging to the S. enterica genus. Worldwide, it is one of the most frequent causes of food borne disease. Similar to S. Typhimurium, some virulence genes of S. Enteritidis are located in pathogenicity islands and prophages. In this study we have generated a mutant strain of S. Enteritidis lacking a prophage-like element, denominated varphiSE12. The resulting mutant strain was attenuated and promoted protective immunity in infected mice. Although S. Enteritidis strains lacking the complete prophage varphiSE12 remained capable of surviving inside phagocytic cells, they showed a significantly reduced capacity to colonize internal organs and failed to cause lethal disease in mice. Consistent with these data, infection with S. Enteritidis strains lacking prophage varphiSE12 promoted the production of anti-Salmonella IgG antibodies and led to protection against a challenge with virulent strains of S. Enteritidis. These results suggest that strains lacking this prophage can induce a protective immunity in mice and be considered as potential attenuated vaccines against S. Enteritidis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
1_ASSA2030
/
2_ODS3
/
3_ND
Problema de salud:
1_doencas_transmissiveis
/
2_enfermedades_transmissibles
/
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Salmonella enteritidis
/
Infecciones por Salmonella
/
Secuencia de Bases
/
Eliminación de Secuencia
/
Vacunas contra la Salmonella
/
Profagos
/
Factores de Virulencia
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
Vaccine
Año:
2010
Tipo del documento:
Article
País de afiliación:
Chile