Isolation of mutants defective in early steps of meiotic recombination in the yeast Saccharomyces cerevisiae.
Genetics
; 128(1): 79-88, 1991 May.
Article
en En
| MEDLINE
| ID: mdl-2060778
ABSTRACT
Using a selection based upon the ability of early Rec- mutations (e.g., rad50) to rescue the meiotic lethality of a rad52 spo13 strain, we have isolated 177 mutants. Analysis of 56 of these has generated alleles of the known Rec genes SPO11, ME14 and MER1, as well as defining five new genes REC102, REC104, REC107, REC113 and REC114. Mutations in all of the new genes appear to specifically affect meiosis; they do not have any detectable mitotic phenotype. Mutations in REC102, REC104 and REC107 reduce meiotic recombination several hundred fold. No alleles of RED1 or HOP1 were isolated, consistent with the proposal that these genes may be primarily involved with chromosome pairing and not exchange.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Recombinación Genética
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Saccharomyces cerevisiae
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Genes Fúngicos
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Meiosis
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Mutación
Idioma:
En
Revista:
Genetics
Año:
1991
Tipo del documento:
Article