Mitochondrial DNA transmitted from sperm in the blue mussel Mytilus galloprovincialis showing doubly uniparental inheritance of mitochondria, quantified by real-time PCR.
Zoolog Sci
; 27(7): 611-4, 2010 Jul.
Article
en En
| MEDLINE
| ID: mdl-20608851
ABSTRACT
Doubly uniparental inheritance (DUI) of mitochondrial DNA transmission to progeny has been reported in the mussel, Mytilus. In DUI, males have both paternally (M type) and maternally (F type) transmitted mitochondrial DNA (mtDNA), but females have only the F type. To estimate how much M type mtDNA enters the egg with sperm in the DUI system, ratios of M type to F type mtDNA were measured before and after fertilization. M type mtDNA content in eggs increased markedly after fertilization. Similar patterns in M type content changes after fertilization were observed in crosses using the same males. To compare mtDNA quantities, we subsequently measured the ratios of mtDNA to the 28S ribosomal RNA gene (an endogenous control sequence) in sperm or unfertilized eggs using a real-time polymerase chain reaction (PCR) assay. F type content in unfertilized eggs was greater than the M type in sperm by about 1000-fold on average. M type content in spermatozoa was greater than in unfertilized egg, but their distribution overlapped. These results may explain the post-fertilization changes in zygotic M type content. We previously demonstrated that paternal and maternal M type mtDNAs are transmitted to offspring, and hypothesized that the paternal M type contributed to M type transmission to the next generation more than the maternal type did. These quantitative data on M and F type mtDNA in sperm and eggs provide further support for that hypothesis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN Mitocondrial
/
Reacción en Cadena de la Polimerasa
/
Mytilus
/
Herencia Extracromosómica
/
Mitocondrias
Límite:
Animals
Idioma:
En
Revista:
Zoolog Sci
Asunto de la revista:
BIOLOGIA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Japón