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Ultrastructural localization of extracellular matrix proteins of the lymph node cortex: evidence supporting the reticular network as a pathway for lymphocyte migration.
Sobocinski, Gregg P; Toy, Katherine; Bobrowski, Walter F; Shaw, Stephen; Anderson, Arthur O; Kaldjian, Eric P.
Afiliación
  • Sobocinski GP; MCDB Dept, University of Michigan, Ann Arbor, MI, USA.
BMC Immunol ; 11: 42, 2010 Aug 17.
Article en En | MEDLINE | ID: mdl-20716349
ABSTRACT

BACKGROUND:

The lymph node (LN) is a crossroads of blood and lymphatic vessels allowing circulating lymphocytes to efficiently recognize foreign molecules displayed on antigen presenting cells. Increasing evidence indicates that after crossing high endothelial venules, lymphocytes migrate within the node along the reticular network (RN), a scaffold of fibers enwrapped by fibroblastic reticular cells (FRC). Light microscopy has shown that the RN contains specific extracellular matrix (ECM) proteins, which are putative molecular "footholds" for migration, and are known ligands for lymphocyte integrin adhesion receptors.

RESULTS:

To investigate whether ECM proteins of the RN are present on the outer surface of the FRC and are thus accessible to migrating lymphocytes, ultrastructural immunohistochemical staining of cynomolgus monkey LN was performed using antibodies to human ECM proteins that were successfully employed at the light microscopic level. The fibrillar collagens I and III were observed primarily within the reticular network fibers themselves. In contrast, the matrix proteins laminin, fibronectin, collagen IV, and tenascin were observed within the reticular fibers and also on the outer membrane surface of the FRC.

CONCLUSIONS:

These findings suggest a molecular basis for how the RN functions as a pathway for lymphocyte migration within the lymph node.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reticulina / Proteínas de la Matriz Extracelular / Colágeno Tipo I / Colágeno Tipo III / Ganglios Linfáticos Límite: Animals / Female / Humans Idioma: En Revista: BMC Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2010 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reticulina / Proteínas de la Matriz Extracelular / Colágeno Tipo I / Colágeno Tipo III / Ganglios Linfáticos Límite: Animals / Female / Humans Idioma: En Revista: BMC Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2010 Tipo del documento: Article País de afiliación: Estados Unidos
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