Jacalin-unbound fraction of Taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid.
Diagn Microbiol Infect Dis
; 68(3): 259-64, 2010 Nov.
Article
en En
| MEDLINE
| ID: mdl-20846809
ABSTRACT
The aim of this study was to evaluate jacalin-bound fraction (JBF) and jacalin-unbound fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose(®)-jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39-42, 47-52, 64-68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_helminthiasis
/
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Parasitología
/
Líquido Cefalorraquídeo
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Neurocisticercosis
/
Taenia saginata
/
Lectinas de Plantas
/
Antígenos Helmínticos
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Adult
/
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Año:
2010
Tipo del documento:
Article
País de afiliación:
Brasil