Isozyme analysis and relationships among three species in Malaysian Trichoderma isolates.
J Microbiol Biotechnol
; 20(9): 1266-75, 2010 Sep.
Article
en En
| MEDLINE
| ID: mdl-20890090
ABSTRACT
Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total protein patterns were shown to be useful for the detection of three Trichoderma species. The isozyme and protein data were analyzed using the Nei and Li Dice similarity coefficient for pairwise comparison between individual isolates, species isolate group, and for generating a distance matrix. The UPGMA cluster analysis showed a higher degree of relationship between T. harzianum and T. aureoviride than to T. longibrachiatum. These results suggested that the T. harzianum isolates had high levels of genetic variation compared to the other isolates of Trichoderma species.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Trichoderma
/
Proteínas Fúngicas
País/Región como asunto:
Asia
Idioma:
En
Revista:
J Microbiol Biotechnol
Año:
2010
Tipo del documento:
Article
País de afiliación:
Malasia