An approach for differentiating uniform glutamatergic neurons from mouse embryonic stem cells.
Anal Biochem
; 410(1): 149-51, 2011 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-21094123
Much effort is being marshaled to generate uniform neuronal populations from embryonic stem (ES) cells, but a completely reliable method has yet to be developed. Here we modified and established a method that brings us closer to this goal. By examining many parameters, we found that the optimal timing of applying a freshly made trypsin/EDTA (ethylenediaminetetraacetic acid) solution to dissociate embryoid bodies determines the success of the outcome. Analyses demonstrated that with this approach, more than 87% of cells differentiated into glutamatergic neurons. Hence, these uniform neurons that were differentiated from ES cells provide an ideal cellular model for many aspects of research.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
/
Técnicas Citológicas
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Ácido Glutámico
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Células Madre Embrionarias
/
Neuronas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Biochem
Año:
2011
Tipo del documento:
Article
País de afiliación:
Taiwán