[The expression and control of telomerase in preantral ovary].

ABSTRACT

AIM: To study the expression and control of telomerase in rat preantral ovary. METHODS: Added different factors to the preantral ovarian granulosa cells, then using TRAP-ELISA to analyze the expression of telomerase. RESULTS: Telomerase activation was detected in granulosa cells. Telomerase activation could be significantly enhanced by hcG, FSH, verapamil and dbcAMP, and it was significantly reduced by antisense-c-myb ODN treatment. We also used radioimmunoassay to determine proterone and estrogen in these cell culture mediums. It was found that these two hormones' secretion were raised under verapamil and FSH; no change under hcG and dbcAMllted with telomerase activity. In MTT assay, the antisense hTERT ODN could significantly inhibited the proliferation of ovarian granulosa cells. CONCLUSION: It is suggested that telomerase activation is present in ovary antral granulosa cell. Anti-c-myb might inhibit telomerase activation, while FSH, hcG, verapamil, dbcAMP might enhance the telomerase activation.