Selection of peptides binding to HCV e2 and inhibiting viral infectivity.
J Microbiol Biotechnol
; 20(12): 1769-71, 2010 Dec.
Article
en En
| MEDLINE
| ID: mdl-21193836
ABSTRACT
The envelope glycoprotein E2 of hepatitis C virus (HCV) binds to various cell surface receptors for viral infection. We performed biopanning against this protein and selected peptides from phage display peptide libraries. Two short peptides, pep7-1 and pep12-1, were selected and their ability to inhibit the infection process was investigated. When pep7-1 was present, the infectivity of HCV particles in cell culture was notably decreased. This decrease was demonstrated by Western blot analysis, immunofluorescence assay, and reverse transcription PCR assay. However, pep12-1 showed little inhibitory effect on HCV infection.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antivirales
/
Péptidos
/
Proteínas del Envoltorio Viral
/
Hepacivirus
/
Internalización del Virus
Límite:
Humans
Idioma:
En
Revista:
J Microbiol Biotechnol
Año:
2010
Tipo del documento:
Article