Serial line scan encoding imaging cytometer for both adherent and suspended cells.
Anal Chem
; 83(5): 1587-93, 2011 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-21291176
ABSTRACT
We present a new design of an imaging cytometer for high content bioanalysis, which is equipped with a low-cost linear complementary metal oxide semiconductor (CMOS) imager (running at ≥40kHz). The fluorescent signals are encoded in a series of line scans across the cellular body, while it streams through a precisely defined line-shaped focus spot. This bioanalysis platform enables the concurrent collection of multiple fluorescence channels, while maintaining both high resolution and excellent throughput (1000 cells/s). We develop several image processing routines for the on-the-fly quantitative analysis of subcellular structures. Finally, we characterize our prototype system by imaging both adherent cells (plate format) and suspended cells (microfluidics format).
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Citometría de Flujo
Idioma:
En
Revista:
Anal Chem
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos