Infection of primary human tonsillar lymphoid cells by KSHV reveals frequent but abortive infection of T cells.
Virology
; 413(1): 1-11, 2011 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-21353276
ABSTRACT
The lymphotropic herpesvirus KSHV principally infects B cells in vivo and is linked to several human B cell lymphoproliferative syndromes. Here we examine the susceptibility of primary tonsillar lymphocytes to infection by a recombinant KSHV (rKSHV.219) that constitutively expresses GFP. At an MOI of ~1, ca. 5-10% of CD19+ B cells became GFP-positive. Surprisingly, in the same culture many more T cells became infected. However, in contrast to isolated B cells, isolated infected T cells did not support correct viral transcription and did not produce infectious virus, indicating the presence of one or more post-entry blocks to lytic KSHV replication in T cells. No immortalization or transformation has yet been observed in either B or T cells. These results affirm the feasibility of studying KSHV infection in primary lymphoid cells, and help to rationalize the detection of KSHV DNA in rare human T cell lymphomas in vivo.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tonsila Palatina
/
Linfocitos
/
Linfocitos T
/
Infecciones por Herpesviridae
/
Herpesvirus Humano 8
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Virology
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos