Expression of hepatic mitochondrial carbonic anhydrase V.
Biochim Biophys Acta
; 1036(3): 237-41, 1990 Dec 06.
Article
en En
| MEDLINE
| ID: mdl-2147861
ABSTRACT
We have raised specific (rabbit anti-rat) polyclonal antibodies to hepatic mitochondrial carbonic anhydrase V (CA V) and used them to assay the amounts of protein expressed in liver mitochondria isolated from term-foetal, control or diabetic adult rats and in perivenous and periportal rat hepatocytes. The levels of CA V expressed in mitochondria isolated from the livers of adult male and female rats are similar and increase (about 2-fold) in mitochondria from adult diabetic rats when compared to those isolated from the livers of control rats. The level of enzyme in adult liver was higher than in the livers of term-foetal rats. CA V is expressed in both perivenous and periportal hepatocytes, but the level of expression is greater (approx. 40%) in perivenous cells. The implications and significance of these findings are discussed with reference to the roles and properties of the other carbonic anhydrase isoenzymes and the metabolic function of the mitochondrial isoenzyme.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Mitocondrias Hepáticas
/
Anhidrasas Carbónicas
/
Diabetes Mellitus Experimental
/
Isoenzimas
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1990
Tipo del documento:
Article
País de afiliación:
Reino Unido