Monoclonal antibodies against Muscleblind-like 3, a protein with punctate nuclear localization.
Hybridoma (Larchmt)
; 30(2): 181-8, 2011 Apr.
Article
en En
| MEDLINE
| ID: mdl-21529292
ABSTRACT
Muscleblind-like 3 (MBNL3) belongs to a family of RNA binding proteins that regulate alternative splicing. We have generated a set of monoclonal antibodies (MAbs) against mouse MBNL3, three of which do not cross-react with the other muscleblind-like (MBNL) proteins, MBNL1 and MBNL2. Epitope mapping revealed that MAbs P1C7, P1E7, SP1C2, and P2E6 recognize distinct, non-overlapping segments of the MBNL3 polypeptide sequence. Immunohistochemical staining of proliferating muscle precursor cells localized MBNL3 to the nucleus in a punctate pattern, characteristic of subcellular structures in the nucleus enriched in pre-messenger RNA splicing factors. Although MBNL3 did not co-localize with SC35 and PSP1 (widely used markers of splicing speckles and paraspeckles), the punctate localization pattern of MBNL3 within interchromatin regions of the nucleus is highly predictive of proteins involved in pre-mRNA processing. Monoclonal antibodies specific for mouse MBNL3 will facilitate further investigation of the expression pattern and unique functions of this splicing factor during development and in different adult mouse tissues.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
2_ODS3
/
3_ND
Problema de salud:
2_enfermedades_transmissibles
/
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Proteínas Recombinantes
/
Proteínas Portadoras
/
Regulación del Desarrollo de la Expresión Génica
/
Isoformas de Proteínas
/
Mioblastos
/
Anticuerpos Monoclonales
/
Especificidad de Anticuerpos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Hybridoma (Larchmt)
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos