Development and characterization of microsatellite markers for Sagittaria trifolia var. sinensis (Alismataceae).
Am J Bot
; 98(2): e36-8, 2011 Feb.
Article
en En
| MEDLINE
| ID: mdl-21613103
ABSTRACT
PREMISE OF THE STUDY Microsatellite markers were developed for the aquatic plant Sagittaria trifolia var. sinensis to assess its genetic diversity and population structure. Cross-species transferability was assayed in eight congeneric species. METHODS AND RESULTS:
Seventeen microsatellite markers were isolated and characterized in Sagittaria trifolia var. sinensis using Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. Across the evaluated populations, 14 of the markers showed polymorphisms with 3 to 11 alleles per locus; the observed and expected heterozygosity (H(o) and H(E)) ranged from 0.0000 to 0.6364 and from 0.0000 to 0.8386, respectively. Nine of the loci were successfully amplified in the congeneric species.CONCLUSIONS:
These markers will be useful for further investigation of population genetics in Sagittaria trifolia var. sinensis and related research in Sagittaria species.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polimorfismo Genético
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ADN de Plantas
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Repeticiones de Microsatélite
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Sagittaria
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Alelos
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Sitios Genéticos
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Heterocigoto
Idioma:
En
Revista:
Am J Bot
Año:
2011
Tipo del documento:
Article