Comparative analysis of microbial DNA extraction protocols for groundwater samples.
Anal Biochem
; 416(2): 240-2, 2011 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-21683680
ABSTRACT
A comparative analysis of four different DNA extraction protocols was performed to determine the best choice for groundwater microbial diversity studies using temperature gradient gel electrophoresis (TGGE) analysis. The methods used were a chelex-based method, a modified salting out procedure (MSOP), and the commercial kits Epicentre and FastDNA. Both commercial kits exhibited the greatest reproducibility in their methods; however, their band patterns were very different. The protocol that showed the highest diversity was the chelex-based method, and the one that showed the lowest diversity was the FastDNA kit.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Electroforesis en Gel de Gradiente Desnaturalizante
/
Agua Dulce
Idioma:
En
Revista:
Anal Biochem
Año:
2011
Tipo del documento:
Article
País de afiliación:
España