Molecular identification and barcodes for the genus Nymphaea.
Acta Biol Hung
; 62(3): 328-40, 2011 Sep.
Article
en En
| MEDLINE
| ID: mdl-21840834
ABSTRACT
Nymphaea species, the most popular decorative plants, were collected for specificity of inter-simple sequence repeat (ISSR) analyses in species identification and differentiation of cultivars and natural populations. Dendrogram constructed from ISSR analyses separated out wild species, namely Nymphaea cyanea, N. nouchali, N. capensis, N. lotus and an outgroup N. mexicana, and cultivars. The dendrogram indicates that the cultivars should be differentiated from N. capensis, as they are sister individuals of N. capensis. The ISSR banding data and the dendrogram are concordantly concluded that wild N. capensis would be an effective type species for producing different cultivars. After plant identification by ISSR markers, DNA barcodes of all sample materials were done to provide species specific markers which can be used for rapid and accurate further plant identification without morphological characters. DNA barcoding sequence analysis indicates genetic distance values. All sequences were recorded in GenBank database.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Nymphaea
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Acta Biol Hung
Año:
2011
Tipo del documento:
Article
País de afiliación:
Tailandia