Enhanced urothelial expression of human chorionic gonadotropin beta (hCGß) in bladder pain syndrome/interstitial cystitis (BPS/IC).
World J Urol
; 30(3): 411-7, 2012 Jun.
Article
en En
| MEDLINE
| ID: mdl-21877171
ABSTRACT
PURPOSE:
Bladder pain syndrome/interstitial cystitis (BPS/IC) is associated with urothelial lesions. Pathomechanisms of urothelial damage and factors for urothelial restoration are unknown. hCG is a factor for cellular differentiation, angiogenesis and immune competence of the endometrium during pregnancy. Clinical observations demonstrate improvement of BPS/IC symptoms during pregnancy or during infertility treatment with hCG. Our research aims were to examine the expression of hCG and luteinizing hormone receptor (LHR) in the urothelium of BPS/IC patients and compare the levels of hCGß with healthy controls.METHODS:
Bladder biopsies of BPS/IC (CLSM n = 10; qPCR n = 15); Tumour-free control tissue from cystectomies (n = 12). hCGα, hCGß and LHR expression were examined by confocal laser scanning microscopy (CLSM), and hCGß expression was quantified. hCGß5 and hCGß7 mRNA splice variants were quantified in real-time polymerase chain reaction.RESULTS:
We found constitutive expression of hCGα, hCGß and LHR in healthy controls. HCGß was significantly upregulated in BPS/IC patients in CLSM. PCR analysis revealed higher levels of hCGß7 than hCGß5 in controls and BPS/IC patients.CONCLUSIONS:
The constitutive expression of hCG and LHR speaks in favour for a functional signalling in urothelial cells without any association with either pregnancy or tumour. We show for the first time that hCGß is upregulated in BPS/IC urothelium and that hCGß7 is the dominant splice variant in those cells. Our findings imply a major role of hCG for urothelial integrity and a disturbance of hCG signalling in case of BPS/IC. We conclude that hCG could gain therapeutical relevance in the future.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Regulación hacia Arriba
/
Cistitis Intersticial
/
Gonadotropina Coriónica Humana de Subunidad beta
/
Urotelio
Tipo de estudio:
Observational_studies
/
Risk_factors_studies
Límite:
Aged
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
World J Urol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Alemania