In vivo gene transfer into the ocular ciliary muscle mediated by ultrasound and microbubbles.
Ultrasound Med Biol
; 37(11): 1814-27, 2011 Nov.
Article
en En
| MEDLINE
| ID: mdl-21963032
ABSTRACT
This study aimed to assess application of ultrasound (US) combined with microbubbles (MB) to transfect the ciliary muscle of rat eyes. Reporter DNA plasmids encoding for Gaussia luciferase, ß-galactosidase or the green fluorescent protein (GFP), alone or mixed with 50% Artison MB, were injected into the ciliary muscle, with or without US exposure (US set at 1 MHz, 2 W/cm(2), 50% duty cycle for 2 min). Luciferase activity was measured in ocular fluids at 7 and 30 days after sonoporation. At 1 week, the US+MB treatment showed a significant increase in luminescence compared with control eyes, injected with plasmid only, with or without MB (×2.6), and, reporter proteins were localized in the ciliary muscle by histochemical analysis. At 1 month, a significant decrease in luciferase activity was observed in all groups. A rise in lens and ciliary muscle temperature was measured during the procedure but did not result in any observable or microscopic damages at 1 and 8 days. The feasibility to transfer gene into the ciliary muscle by US and MB suggests that sonoporation may allow intraocular production of proteins for the treatment of inflammatory, angiogenic and/or degenerative retinal diseases.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sonicación
/
Cuerpo Ciliar
/
Técnicas de Transferencia de Gen
Límite:
Animals
Idioma:
En
Revista:
Ultrasound Med Biol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Francia