Noninvasive diagnosis of mucopolysaccharidosis via depth-resolved optical spectroscopy of the outer ear.
Biomed Opt Express
; 2(10): 2741-8, 2011 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-22025980
ABSTRACT
Current diagnostics for lysosomal storage disorders such as mucopolysaccharidosis (MPS) rely on evaluation of ex vivo bodily fluids, which has several shortcomings. In this study, we evaluated whether Raman spectroscopy could noninvasively diagnose MPS in a murine model. Via confocal sampling of the murine outer ear, Raman spectra were obtained at multiple depths. Partial least-squares discriminant analysis of the processed Raman spectra showed a 93% sensitivity and 91% specificity for disease. The discriminant algorithm relied on several Raman bands related to glycosaminoglycans (GAGs) that typically accumulate in MPS. These findings indicate the possibility for a new, noninvasive diagnostic tool for MPS.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Biomed Opt Express
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos