Attachment of anti-GFP antibodies to microspheres for optical trapping experiments.
Cold Spring Harb Protoc
; 2011(11): 1370-1, 2011 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-22046049
ABSTRACT
In vitro motility assays enabled the analysis of coupling between ATP hydrolysis and movement of myosin along actin filaments or kinesin along microtubules. Single-molecule assays using laser trapping have been used to obtain more detailed information about kinesins, myosins, and processive DNA enzymes. The combination of in vitro motility assays with laser-trap measurements has revealed detailed dynamic structural changes associated with the ATPase cycle. This protocol describes a method for attaching anti-GFP (green fluorescent protein) antibodies to microspheres. GFP-motor fusion proteins can then be adsorbed to the microspheres for use in single-molecule motility studies and optical trapping experiments.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Fluorescentes Verdes
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Pinzas Ópticas
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Microesferas
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Anticuerpos
Idioma:
En
Revista:
Cold Spring Harb Protoc
Año:
2011
Tipo del documento:
Article