The optical trapping dumbbell assay for nonprocessive motors or motors that turn around filaments.
Cold Spring Harb Protoc
; 2011(11): 1372-4, 2011 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-22046050
ABSTRACT
In vitro motility assays enabled the analysis of coupling between ATP hydrolysis and movement of myosin along actin filaments or kinesin along microtubules. Single-molecule assays using laser trapping have been used to obtain more detailed information about kinesins, myosins, and processive DNA enzymes. The combination of in vitro motility assays with laser-trap measurements has revealed detailed dynamic structural changes associated with the ATPase cycle. This protocol describes the preparation of biotin-actin filaments and coverslips coated with polystyrene beads. These are then used in optical trapping dumbbell assays to study interactions between motors and filaments.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biotina
/
Citoesqueleto de Actina
/
Proteínas Motoras Moleculares
/
Pinzas Ópticas
Idioma:
En
Revista:
Cold Spring Harb Protoc
Año:
2011
Tipo del documento:
Article