Molar absorptivities of bilirubin (NIST SRM 916a) and its neutral and alkaline azopigments.
Clin Chem
; 36(9): 1698-701, 1990 Sep.
Article
en En
| MEDLINE
| ID: mdl-2208723
ABSTRACT
Three laboratories in the U.S. and two in the Netherlands determined molar absorptivities (epsilon) of Standard Reference Material (SRM) 916a Bilirubin from the National Institute of Standards and Technology. In caffeine reagent the average epsilon values were 50,060 and 48, 980 L.mol-1.cm-1 at 432 and 457 nm, respectively. The epsilon value of the blue azopigment, obtained with the Reference Method for total serum bilirubin, was 76,490 L.mol-1.cm-1 at 598 nm. When the addition of alkaline tartrate was omitted, the molar absorptivity of the red azopigment was 56,600 L.mol-1.cm-1 at 530 nm.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos Azo
/
Bilirrubina
/
Química Clínica
Límite:
Humans
Idioma:
En
Revista:
Clin Chem
Asunto de la revista:
QUIMICA CLINICA
Año:
1990
Tipo del documento:
Article