Successful propagation of Alkhumra (misnamed as Alkhurma) virus in C6/36 mosquito cells.
Trans R Soc Trop Med Hyg
; 106(3): 180-5, 2012 Mar.
Article
en En
| MEDLINE
| ID: mdl-22154975
ABSTRACT
Epidemiological data suggest that Alkhumra (misnamed as Alkhurma) virus (ALKV) is transmitted from livestock animals to humans by direct contact with animals or by the mosquito bites, but not by ticks. To assess the ability of the virus to replicate in mosquito cells, serum and plasma of seven acutely febrile patients with clinically suspected ALKV infection reported in Najran, Saudi Arabia in 2009 were inoculated onto Aedes albopictus mosquito cells (C6/36) and directly examined with ALKV-RNA-specific real time RT-PCR as well as indirect immunfluorescence assay (IFA) using ALKV-specific polyclonal antibodies. The isolated virus was titrated in the mammalian rhesus monkey kidney cells (LLC-MK2). Five of the seven specimens were RT-PCR- and culture-positive demonstrating cytopathic effects in the form of cell rounding and aggregation appearing on day 3 post inoculation with syncytia eventually appearing on day 8 post inoculation. Identification of ALKV-RNA in the cell culture was confirmed with RT-PCR and IFA. The virus titre was 3.2×10(6) tissue culture infective dose 50 (TCID(50)) per mL. Three more viral passages were successfully made in the C6/36 cells. This is the first description of propagation of ALKV in mosquito cells.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_dengue
Asunto principal:
ARN Viral
/
Infecciones por Flavivirus
/
Aedes
/
Flavivirus
/
Fiebres Hemorrágicas Virales
Límite:
Animals
/
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Trans R Soc Trop Med Hyg
Año:
2012
Tipo del documento:
Article
País de afiliación:
Arabia Saudita