TaqMan real-time PCR for detection and quantitation of squash leaf curl virus in cucurbits.
J Virol Methods
; 179(2): 367-72, 2012 Feb.
Article
en En
| MEDLINE
| ID: mdl-22172970
ABSTRACT
A real-time PCR assay based on the TaqMan chemistry was developed for reliable detection and quantitation of the squash leaf curl virus (SLCV) in melon and squash plants. This method was highly specific to SLCV and it was about one thousand times more sensitive than the conventional PCR method. The protocol of the real-time PCR established in this study enabled detection of as little as 10(2) copies of SLCV DNA with CP gene as the target. This TaqMan real-time PCR assay for detection and quantitation of SLCV would be a useful tool for application in quarantine and certification of SLCV in cucurbits as well as in the research of disease resistance and epidemiology.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de las Plantas
/
Virología
/
Cucurbita
/
Begomovirus
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
J Virol Methods
Año:
2012
Tipo del documento:
Article
País de afiliación:
Taiwán