Antitumor activity from the combined application of poly(I:C) and chemotherapeutics in human metastatic pharyngeal cell lines.
Chemotherapy
; 57(6): 460-7, 2011.
Article
en En
| MEDLINE
| ID: mdl-22188667
ABSTRACT
BACKGROUND:
Toll-like receptor 3 (TLR3) activation in tumor cells induces apoptosis. We investigated the effect of TLR3 ligand (poly(IC)) in combination with chemotherapeutics applied to human pharyngeal carcinoma cells as a possible antitumor therapy.METHODS:
Human pharyngeal cancer cell lines were studied (FaDu and Detroit 562). Cytotoxicity assays and apoptosis assays (annexin V staining and caspase 3/7 activity measurements) were used to investigate the cytotoxic effects. By using TLR3 siRNA we confirmed that the observed effect is TLR3-dependent.RESULTS:
We found that the combined application of poly(IC) and chemotherapeutics (cisPt, HU, 5-FU and MTX) has a stronger inhibitory effect on cell growth in tumor cells expressing functional TLR3 as compared with a single treatment. This is a result of TLR3-dependent apoptosis.CONCLUSION:
Our study showed that a combined application of the two agents already being used in tumor therapy could lower the necessary dosage of chemotherapeutics, leading to fewer side effects.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Protocolos de Quimioterapia Combinada Antineoplásica
/
Neoplasias Faríngeas
Límite:
Humans
Idioma:
En
Revista:
Chemotherapy
Año:
2011
Tipo del documento:
Article
País de afiliación:
Croacia