ANCUT2, an extracellular cutinase from Aspergillus nidulans induced by olive oil.
Appl Biochem Biotechnol
; 166(5): 1275-90, 2012 Mar.
Article
en En
| MEDLINE
| ID: mdl-22238011
ABSTRACT
Cutinases are versatile carboxylic ester hydrolases with great potential in many biocatalytic processes, including biodiesel production. Genome sequence analysis of the model organism Aspergillus nidulans reveals four genes encoding putative cutinases. In this work, we purified and identified for the first time a cutinase (ANCUT2) produced by A. nidulans. ANCUT2 is a 29-kDa protein which consists of 255 amino acid residues. Comparison of the amino acid sequence of ANCUT2 with other microbial cutinase sequences revealed a high degree of homology with other fungal cutinases as well as new features, which include a serine-rich region and conserved cysteines. Cutinase production with different lipidic and carbon sources was also explored. Enzyme activity was induced by olive oil and some triacylglycerides and fatty acids, whereas it was repressed by glucose (1%) and other sugars. In some conditions, a 22-kDa post-translational processing product was also detected. The cutinase nature of the enzyme was confirmed after degradation of apple cutin.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aspergillus nidulans
/
Aceites de Plantas
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Hidrolasas de Éster Carboxílico
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Espacio Extracelular
Idioma:
En
Revista:
Appl Biochem Biotechnol
Año:
2012
Tipo del documento:
Article
País de afiliación:
México